Flow Cytometric Reticulocyte Counting
نویسندگان
چکیده
منابع مشابه
Reticulocyte counting using flow cytometry.
A flow cytometric method for the quantitation of reticulocytes was refined for routine laboratory use. Blood (2 microliters) is added to 2 ml of 0.4 microM thiazole orange in phosphate buffered saline, incubated at room temperature for 90 minutes, and analysed on a Coulter EPICS Profile flow cytometer, with gating for red cells on the basis of forward and right angled light scatter. Blood (2 mi...
متن کاملFlow cytometric reticulocyte counting. Parallel evaluation of five fully automated analyzers: an NCCLS-ICSH approach.
We performed a parallel evaluation of 5 automated reticulocyte analyzers. The guidelines were those proposed by the National Committee for Clinical Laboratory Standards and the International Council for Standardisation in Haematology. Duplicate analyses were performed for 225 healthy subjects and 115 patients affected by various diseases. The reference intervals were different for each method (...
متن کاملOptimized Method for Reticulocyte Counting: Simple, Accurate, and Comparable to Flow Cytometry
Background: Reticulocytes are immature red blood cells with RNA, spending the final stages of their maturation in the peripheral blood. The number of reticulocytes in the peripheral blood is the salient evidence of the effectiveness of bone marrow to produce red blood cells. Currently, reticulocyte count is done manually or automatically in clinical laboratories. Difficulties and limitations of...
متن کاملReticulocyte counting by flow cytometry. A comparison with manual methods.
The reticulocyte count (RC) is a key diagnostic test in the evaluation, classification, and response to therapy of anemia. The RC, as determined by manual methods, has a frustrating inherent imprecision owing to its binomial counting statistics (i.e., low counts/low precision) and inaccuracy because of inter- and intraobserver variability as to what indeed is a reticulocyte. Fluorescent activat...
متن کاملErratum: Flow Cytometric White Blood Cell Differential Using CytoDiff is Excellent for Counting Blasts
BACKGROUND The usefulness of the CytoDiff flow cytometric system (Beckman Coulter, USA) has been studied in various conditions, but its performance including rapidity in detecting and counting blasts, the most significant abnormal cells in the peripheral blood, has not been well evaluated. The objective of this study was to evaluate the performance of the CytoDiff differential counting method i...
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ژورنال
عنوان ژورنال: American Journal of Clinical Pathology
سال: 2001
ISSN: 0002-9173,1943-7722
DOI: 10.1309/m26b-1ynq-vnu8-m1ce